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Eggshell Membrane as a Bioactive Agent in Polymeric Nanotopographic Scaffolds for Enhanced Bone Regeneration
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  • Daum Kim,
  • Yonghyun Gwon,
  • Sunho Park,
  • Sujin Kim,
  • Woochan Kim,
  • Kwidug Yun,
  • Jangho Kim
Daum Kim
Chonnam National University
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Yonghyun Gwon
Chonnam National University
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Sunho Park
Chonnam National University
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Sujin Kim
Chonnam National University
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Woochan Kim
Chonnam National University
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Kwidug Yun
Chonnam National University
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Jangho Kim
Chonnam National University
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Abstract

A bone regeneration scaffold is typically designed as a platform to effectively heal a bone defect while preventing soft tissue infiltration. Despite the wide variety of scaffold materials currently available, such as collagen, critical problems in achieving bone regeneration remain, including a rapid absorption period and low tensile strength as well as high costs. Inspired by extracellular matrix protein and topographical cues, we developed a polycaprolactone-based scaffold for bone regeneration using a soluble eggshell membrane protein (SEP) coating and a nanotopography structure for enhancing the physical properties and bioactivity. The scaffold exhibited adequate flexibility and mechanical strength as a biomedical platform for bone regeneration. The highly aligned nanostructures and SEP coating were found to regulate and enhance cell morphology, adhesion, proliferation, and differentiation in vitro. In a calvarial bone defect mouse model, the scaffolds coated with SEP applied to the defect site promoted bone regeneration along the direction of the nanotopography in vivo. These findings demonstrate that bone-inspired nanostructures and SEP coatings have high potential to be applicable in the design and manipulation of scaffolds for bone regeneration.

Peer review status:Published

09 Sep 2020Submitted to Biotechnology and Bioengineering
18 Sep 2020Submission Checks Completed
18 Sep 2020Assigned to Editor
10 Oct 2020Reviewer(s) Assigned
21 Nov 2020Review(s) Completed, Editorial Evaluation Pending
21 Nov 2020Editorial Decision: Revise Minor
19 Jan 20211st Revision Received
20 Jan 2021Submission Checks Completed
20 Jan 2021Assigned to Editor
23 Jan 2021Review(s) Completed, Editorial Evaluation Pending
23 Jan 2021Editorial Decision: Accept
07 Feb 2021Published in Biotechnology and Bioengineering. 10.1002/bit.27702