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Staphylococcus aureus ST228 and ST239 as models for expression studies of diverse markers during osteoblast infection and persistence
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  • Dafne Bongiorno,
  • Nicolò Musso,
  • Giuseppe Caruso,
  • Lorenzo Lazzaro,
  • Filippo Caraci,
  • Stefania Stefani,
  • Floriana Campanile
Dafne Bongiorno
University of Catania
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Nicolò Musso
University of Catania
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Giuseppe Caruso
University of Catania
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Lorenzo Lazzaro
University of Catania
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Filippo Caraci
University of Catania
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Stefania Stefani
University of Catania
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Floriana Campanile
University of Catania
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Abstract

The ability of S. aureus to infect bone and osteoblasts is correlated to its incredible virulence armamentarium that can mediate the invasion/internalization process, cytotoxicity, membrane damage and intracellular persistence. We comparatively analyzed the interaction, persistence and modulation of expression of selected genes as well as cell viability in an ex-vivo model using human MG-63 osteoblasts of two previously studied and well-characterized S. aureus clinical strains belonging to ST239-SCCmecIII-t037 and ST228-SCCmecI-t041 clones at 3h and 24h post-infection (p.i). ATCC12598 was used as a control strain. Using Imaging Flow Cytometry analysis, we found that strains differently invaded osteoblasts after 3h and 24h: ATCC12598 internalized in 70% and 50% of cells, ST239-SCCmecIII in 50% and 45% and ST228-SCCmecI in 30% and 20%, respectively. ST239-III, during the infection period, exerted a significative cytotoxic activity due to the over-expression of hla and psmA and the increased expression of the genes involved in adhesion, probably due to the release and re-entry of bacteria inside MG-63 at 24h p.i. The lower invasiveness of ST228-I was also correlated with the non-cytotoxic activity inside osteoblasts. This clone was not able to activate a sufficient cellular reaction and succumbed in-side the MG-63 cells.

Peer review status:Published

08 Sep 2020Submitted to MicrobiologyOpen
09 Sep 2020Submission Checks Completed
09 Sep 2020Assigned to Editor
24 Sep 2020Reviewer(s) Assigned
21 Oct 2020Review(s) Completed, Editorial Evaluation Pending
21 Oct 2020Editorial Decision: Revise Minor
23 Dec 20201st Revision Received
24 Dec 2020Assigned to Editor
24 Dec 2020Submission Checks Completed
27 Dec 2020Review(s) Completed, Editorial Evaluation Pending
29 Dec 2020Reviewer(s) Assigned
22 Jan 2021Editorial Decision: Revise Minor
25 Jan 20212nd Revision Received
27 Jan 2021Assigned to Editor
27 Jan 2021Submission Checks Completed
27 Jan 2021Review(s) Completed, Editorial Evaluation Pending
13 Feb 2021Editorial Decision: Accept
Mar 2021Published in MicrobiologyOpen volume 10 issue 2. 10.1002/mbo3.1178