loading page

Chromosomal-level genome assembly of silver sillago (Sillago sihama)
  • +10
  • Changxu Tian,
  • Xinghua Lin,
  • Yang Huang,
  • Huapu Chen,
  • Dongneng Jiang,
  • Hongjuan Shi,
  • Siping Deng,
  • Tianli Wu,
  • Yulei Zhang,
  • Mouyan Jiang,
  • Tao Du,
  • Chunhua Zhu,
  • Guangli Li
Changxu Tian
Guangdong Ocean University
Author Profile
Xinghua Lin
Guangdong Ocean University
Author Profile
Yang Huang
Guangdong Ocean University
Author Profile
Huapu Chen
Guangdong Ocean University
Author Profile
Dongneng Jiang
Guangdong Ocean University
Author Profile
Hongjuan Shi
Guangdong Ocean University
Author Profile
Siping Deng
Guangdong Ocean University
Author Profile
Tianli Wu
Guangdong Ocean University
Author Profile
Yulei Zhang
Guangdong Ocean University
Author Profile
Mouyan Jiang
Guangdong Ocean University
Author Profile
Tao Du
Guangdong Ocean University
Author Profile
Chunhua Zhu
Guangdong Ocean University
Author Profile
Guangli Li
Guangdong Ocean University
Author Profile

Abstract

Silver sillago, Sillago sihama is a member of the family Sillaginidae and found in all Chinese inshore waters. It is an emerging commercial marine aquaculture species in China. In this study, high-quality chromosome-level reference genome of S. sihama was first constructed using PacBio Sequel sequencing and high-throughput chromosome conformation capture (Hi-C) technique. A total of 66.16 Gb clean reads were generated by PacBio sequencing platforms. The genome-scale was 521.63 Mb with 556 contigs, and 13.54 Mb of contig N50 length. Additionally, Hi-C scaffolding of the genome resulted in 24 chromosomes containing 96.93 % of the total assembled sequences. A total of 23,959 protein-coding genes were predicted in the genome, and 96.51 % of the genes were functionally annotated in public databases. A total of 71.86 Mb repetitive elements were detected, accounting for 13.78% of the genome. The phylogenetic relationships of silver sillago with other teleosts showed that silver sillago was separated from the common ancestor of S. sinica about 7.92 million years ago. Comparative genomic analysis of silver sillago with other teleosts showed that 45 unique and 100 expansion gene families were identified in silver sillago. Expansion gene families were involved in immune and olfactory receptors. In this study, the genomic resources provide valuable reference genomes for functional genomics research of silver sillago.

Peer review status:UNDER REVIEW

17 Sep 2020Submitted to Molecular Ecology Resources
06 Oct 2020Assigned to Editor
06 Oct 2020Submission Checks Completed
16 Oct 2020Review(s) Completed, Editorial Evaluation Pending