Hybrid observer for high cell density cultures exhibiting overflow
AbstractThis work proposes a hybrid observer based on an asymptotic observer and
an extended Kalman filter applied to S. cerevisiae's high-density
cultures. The observer estimates biomass and ethanol concentrations
based on dissolved oxygen, carbon dioxide, and substrate measurements.
Numerical simulations show that the proposed hybrid observer is useful
for monitoring the respirative and the respiro-fermentative regimes in
high cell density cultivations, showing a robust behavior against
measurement noise. The Monte Carlo simulations allowed the evaluation of
the observer's performance when faced with changes in the plant's
parameters, showing stability in all the evaluated scenarios. The
methodology used in this study can be extended to different cell
factories such as Pichia pastoris and Escherichia coli, modifying the
yields and the kinetic and equilibrium parameters.