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Biomanufacturing of Value-Added Products from Oils or Fats: A Case Study of Yarrowia lipolytica
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  • Na Liu,
  • Ya-Hue Soong,
  • Iman Mirzaee Kakhki,
  • Andrew Olson ,
  • Peng Yu,
  • Hsi-Wu Wong,
  • DONGMING XIE
Na Liu
University of Massachusetts Lowell

Corresponding Author:[email protected]

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Ya-Hue Soong
University of Massachusetts Lowell
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Iman Mirzaee Kakhki
University of Massachusetts Lowell
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Andrew Olson
University of Massachusetts Lowell
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Peng Yu
University of Massachusetts Lowell
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Hsi-Wu Wong
University of Massachusetts Lowell
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DONGMING XIE
University of Massachusetts Lowell
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Abstract

The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that convert waste oils or fats into a series of value-added products. Our research employs the oleaginous yeast Yarrowia lipolytica as a case study for citrate production from waste oils. First, we conducted the CFD simulation of the bioreactor system and identified that the extracellular mixing and mass transfer is the first limiting factor of an oil fermentation process due to the insolubility of oil in water. Based on the CFD simulation results, bioreactor design and operating conditions were optimized and successfully enhanced oil uptake and bioconversion in fed-batch fermentation experiments. After that, we investigated the impacts of cell morphology on oil uptake, intracellular lipid accumulation, and citrate formation by overexpressing and deleting the MHY1 gene in the wild type Y. lipolytica. Fairly good correlations were achieved between cell morphology and productivities of biomass, lipid, and citrate. Finally, fermentation kinetics with both glucose and oil substrates were compared and the oil fermentation process was carefully evaluated. Our research results suggest that waste oils or fats can be economical feedstocks for biomanufacturing of many high-value products.
22 Oct 2020Submitted to Biotechnology and Bioengineering
22 Oct 2020Submission Checks Completed
22 Oct 2020Assigned to Editor
28 Oct 2020Reviewer(s) Assigned
06 Dec 2020Review(s) Completed, Editorial Evaluation Pending
06 Dec 2020Editorial Decision: Revise Major
31 Dec 20201st Revision Received
02 Jan 2021Submission Checks Completed
02 Jan 2021Assigned to Editor
02 Jan 2021Reviewer(s) Assigned
17 Jan 2021Review(s) Completed, Editorial Evaluation Pending
17 Jan 2021Editorial Decision: Accept