Abstract
Since the first report from Cherry Valley ducks on a commercial duck
farm in China (2008), duck astrovirus type 1(DAstV-1) -associated duck
viral hepatitis (DVH) have been detected in several commercial duck
flocks. In the literature, no outbreak of DAstV-1 have been report in
China since 2012. Here, the isolation, cultivation and characterization
of DAstV-1 isolate are described. One DAstV-1 strain, designated as
DAstV-SDZZ, was isolated from a diseased duckling. The isolated
astrovirus grew well in the LMH cell line. To determine the entire
genomic of the DAstV-SDZZ isolate, next-generation sequencing (NGS)
technique was conducted on Illumina HiSeq platform. Complete genome
sequence analysis revealed that DAstV-SDZZ isolate was 91.6%-98.7%
homology with others DAstV-1 deposited in Genbank. Similar clinical
symptoms were successful reproduced by experimental infection study
using the DAstV-SDZZ isolate. DAstV-SDZZ is the first DAstV-1 strain
whose experimental infection study has been conducted. The present works
are likely to provide new insights into the pathogenicity and evolution
of DAstV-1 in ducks.