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Aspergillus awamori endoglucanases promote faster lignocellulosic biomass liquefaction in high-solids enzymatic hydrolysis
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  • Roberta Pereira Espinheira,
  • Vanessa Alves Lima Rocha,
  • Tiago Martins Guimarães,
  • Catarina Amorim Oliveira,
  • Marcella Fernandes de Souza,
  • Gilberto B. Domont,
  • Fábio César Sousa Nogueira,
  • Ricardo Sposina Sobral Teixeira,
  • Elba Pinto da Silva Bon,
  • Ayla Sant'Ana da Silva
Roberta Pereira Espinheira
National Institute of Technology
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Vanessa Alves Lima Rocha
Federal University of Rio de Janeiro
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Tiago Martins Guimarães
National Institute of Technology
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Catarina Amorim Oliveira
Rio de Janeiro Federal Institute of Education Science and Technology
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Marcella Fernandes de Souza
Ghent University
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Gilberto B. Domont
Federal University of Rio de Janeiro
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Fábio César Sousa Nogueira
Federal University of Rio de Janeiro
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Ricardo Sposina Sobral Teixeira
Federal University of Rio de Janeiro
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Elba Pinto da Silva Bon
Federal University of Rio de Janeiro
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Ayla Sant'Ana da Silva
National Institute of Technology
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Abstract

Endoglucanases are necessary to improve high-solids enzymatic hydrolysis of lignocellulosic biomass by promoting liquefaction and decreasing the medium viscosity, alleviating one of the processes’ major hindrances. In this study, endoglucanases produced by a particular strain of Aspergillus awamori were evaluated to speed up biomass liquefaction in reactions with 30% solids. Firstly, A. awamori crude supernatant (Aa) was assessed as a supplement to commercial enzymes, decreasing the media viscosity in 10-fold and improving glucose release by 20% after 24 h. Afterward, Aa was fractionated by size-exclusion chromatography and an endoglucanases-rich fraction was identified by liquid chromatography-mass spectrometry. This fraction was then supplemented to the most efficient commercial enzyme and its performance compared with the unfractionated Aa, resulting in the same improvement on medium viscosity and glucose release in 6 h. These data indicate that A. awamori endoglucanases have a powerful effect on the viscosity decrease during high-solids enzymatic hydrolysis.

Peer review status:POSTED

09 Nov 2020Submitted to Biotechnology and Bioengineering
09 Nov 2020Assigned to Editor
09 Nov 2020Submission Checks Completed