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A lentinan-loaded hydrogel with core-shell structure induces broad-spectrum resistance against plant virus by activating the expression of CML30
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  • Shunyu Xiang,
  • Xue Chen,
  • Jing Wang,
  • Haoran Peng,
  • Xing Lv,
  • Long Jiang,
  • Zhe Cao,
  • Jin Huang,
  • Daibin Wang,
  • Lisong Ma,
  • xianchao sun
Shunyu Xiang
Southwest University
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Xue Chen
Southwest University
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Jing Wang
Southwest University
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Haoran Peng
Southwest University
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Xing Lv
Southwest University
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Long Jiang
Southwest University
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Zhe Cao
Southwest University
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Jin Huang
Southwest University
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Daibin Wang
Chongqing Tobacco Science Research Institute
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Lisong Ma
Agriculture and Agri-Food Canada
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xianchao sun
Southwest University
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Abstract

Control of plant virus disease largely depends on the induced plant defense achieved by the external application of synthetic chemical inducers with the ability to modify defense-signaling pathways. However, most of the molecular mechanisms underlying these inducers remain unknown. Here, we developed a lentinan-loaded hydrogel with the core-shell structure and discovered how it protects plant from different virus infections. The hydrogel was synthesized by adding a chitosan shell on the surface of the sodium alginate-calcium ion-lentinan (LNT) hydrogel (SL-gel) to form CSL-gel. CSL-gel exhibits the capacity of prolonging the stable-release of lentinan and promoting calcium ions release. Application of CSL-gel on the root of plants significantly promotes plant growth and development and induces broad-spectrum resistance against TMV, TuMV, PVX and TRV. Furthermore, we found that the sustained release of calcium ions from the CSL-gel triggers the high expression of cal-modulin-like protein 30 (CML30), and silencing of CML30 enhances the susceptibility of tobacco to TMV. Our findings provide evidence that the novel and synthetic CSL-gel with the sustainable release of LNT and calcium ion strongly inhibits the plant virus infection. This study uncovers a novel mode of action by which CSL-gel with the stable release of calcium ion triggers CML30 expression.