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Arabidopsis sirtuins and poly ADP ribose polymerases regulate gene expression in the day but do not affect circadian rhythms
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  • Jun Hyeok Kim,
  • Laura Bell,
  • Xiao Wang,
  • Rinukshi Wimalasekera,
  • Hugo Bastos,
  • Krystyna Kelly,
  • Matthew Hannah,
  • Alex Webb
Jun Hyeok Kim
University of Cambridge
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Laura Bell
University of Cambridge
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Xiao Wang
University of Cambridge
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Rinukshi Wimalasekera
University of Cambridge
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Hugo Bastos
University of Cambridge
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Krystyna Kelly
University of Cambridge
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Matthew Hannah
Bayer CropScience NV
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Alex Webb
University of Cambridge
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Abstract

Nicotinamide-adenine dinucleotide (NAD) is involved in redox homeostasis and acts as a substrate for NADases, including poly (ADP-ribose) polymerases (PARPs) that add poly (ADP-ribose) polymers to proteins and DNA, and sirtuins that deacetylate proteins. Nicotinamide, a biproduct of NADases increases circadian period in both plants and animals. In mammals, the effect of nicotinamide on circadian period might be mediated by the PARPs and sirtuins because thy directly bind to core circadian oscillator genes. We have investigated whether PARPs and sirtuins contribute to the regulation of the circadian oscillator in Arabidopsis. We found no evidence that PARPs and sirtuins regulate the circadian oscillator of Arabidopsis or are involved in the response to nicotinamide. RNA-seq analysis indicated that PARPs regulate the expression of only a few genes, including FLOWERING LOCUS C. However, we found profound effects of reduced sirtuin 1 expression on gene expression during the day but not at night, and an embryo lethal phenotype in knockouts. Our results demonstrate that PARPS and sirtuins are not associated with NAD regulation of the circadian oscillator and that sirtuin 1 is associated with daytime regulation of gene expression.

Peer review status:Published

10 Nov 2020Submitted to Plant, Cell & Environment
11 Nov 2020Submission Checks Completed
11 Nov 2020Assigned to Editor
18 Nov 2020Reviewer(s) Assigned
08 Dec 2020Review(s) Completed, Editorial Evaluation Pending
08 Dec 2020Editorial Decision: Revise Minor
18 Dec 20201st Revision Received
22 Dec 2020Submission Checks Completed
22 Dec 2020Assigned to Editor
25 Dec 2020Review(s) Completed, Editorial Evaluation Pending
25 Dec 2020Editorial Decision: Accept
19 Jan 2021Published in Plant, Cell & Environment. 10.1111/pce.13996