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The 6th amino acid mutation of Rep protein had no effect on PCV2b but enhanced PCV2d virus replication in vitro
  • +10
  • Xiaoyan Wu,
  • Shuo Wang,
  • Changxun Xin,
  • Chen Li,
  • Jianli Shi,
  • Zhe Peng,
  • Chang Liu,
  • Hong Han,
  • Yao Tian,
  • JiaXin Li,
  • ShaoJian Xu,
  • Jun Li,
  • fan zhang
Xiaoyan Wu
1 Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China
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Changxun Xin
Animal Science and Veterinary Medicine
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Chen Li
Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural
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Jianli Shi
Animal Science and Veterinary Medicine
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Zhe Peng
Animal Science and Veterinary Medicine
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Chang Liu
Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural
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Hong Han
Shandong Academy of Agricultural Sciences
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Yao Tian
Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural
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JiaXin Li
Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural
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ShaoJian Xu
Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural
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Jun Li
Animal Science and Veterinary Medicine
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Abstract

Porcine circovirus type 2 (PCV2) is the etiological agent that primary cause of post-weaning multisystemic wasting syndrome (PMWS). The major genotypes, PCV2a, PCV2b and PCV2d, are highly prevalent, but now replaced with 2b and 2d in swine population in worldwide. Rep protein is the key protein for viral replication. Compared a large number of Rep protein amino acid (aa) sequences, we found that there were three sites with regular changes between 2b and 2d.In order to analyze the effect of key sites on viral replication, we used site-directed mutagenesis to mutate the 6th aa of Rep (alternations with asparagine and serine) between PCV2b and PCV2d, Two wild-type and two mutant viruses infectious clones were rescued by non-contaminated porcine kidney-15 (PK-15) cells. Real-time quantitative PCR and a one-step growth curve were used to determine viral load to assess the replication of rescued viruses. The results showed that there was no significant difference between the PCV2b mutation and the wild-type PCV2b virus in vitro, while the mutation ofPCV2d enhanced viral replication.