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Volatile compounds from beneficial rhizobacteria Bacillus spp. promote periodic lateral root development in Arabidopsis
  • +10
  • Yucong Li,
  • Jiahui Shao,
  • Yuanming Xie,
  • Letian Jia,
  • Yansong Fu,
  • Zhihui Xu,
  • Nan Zhang,
  • Haichao Feng,
  • Weibing Xun,
  • Yunpeng Liu,
  • Qi Rong Shen,
  • Wei Xuan,
  • Ruifu Zhang
Yucong Li
Nanjing Agricultural University
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Jiahui Shao
Nanjing Agricultural University
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Yuanming Xie
Nanjing Agricultural University
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Letian Jia
Nanjing Agricultural University
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Yansong Fu
Nanjing Agricultural University
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Zhihui Xu
Nanjing Agricultural University
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Nan Zhang
Nanjing Agricultural University
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Haichao Feng
Nanjing Agricultural University
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Weibing Xun
Nanjing Agricultural University
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Yunpeng Liu
Chinese Academy of Agricultural Sciences
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Qi Rong Shen
Nanjing Agricultural University
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Wei Xuan
Nanjing Agricultural University
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Ruifu Zhang
Nanjing Agricultural University
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Abstract

Lateral root (LR) formation is coordinated by both endogenous and external factors. As biotic factors, Plant growth-promoting rhizobacteria (PGPRs) can affect LR formation, while the regulation mechanism is unclear. In this study, by applying various marker lines, we found that volatile compounds (VCs) from PGPR strain Bacillus amyloliquefaciens SQR9 induced a high frequency of oscillation and prebranch site formation, and further accelerated the development and emergence of the lateral root primordia (LRP), thus promoting LR development in model plant Arabidopsis. We demonstrated a critical role of auxin on SQR9 VCs-induced lateral root formation via respective mutants and pharmacological experiments. Our results showed that the YUC9 (YUCs)-mediated auxin biosynthesis, polar auxin transport, and auxin signaling pathway are involved in SQR9 VCs-induced LRs formation. We further showed that acetoin, a major component of SQR9 VCs, is less active in promoting root development compared to SQR9 cells, suggesting uncharacterized VCs might contribute to SQR9 effect in mediating LR formation. In summary, our study revealed a novel mechanism of PGPR-produced VCs in regulating LR branching in a non-contact manner, and further efforts will explore useful VCs to promote plant root development.

Peer review status:Published

18 Feb 2021Published in Plant, Cell & Environment. 10.1111/pce.14021