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Modulation of GmFAD3 expression alters responses to abiotic stress in soybean
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  • Ajay Singh,
  • Mahesh Kumar,
  • Susheel Raina,
  • Milind Ratnaparkhe,
  • Jagadish Rane,
  • Rajeev Varshney,
  • Aardra Kachroo
Ajay Singh
National Institute of Abiotic Stress Management
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Mahesh Kumar
National Institute of Abiotic Stress Management
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Susheel Raina
National Bureau of Plant Genetic Resources (ICAR-NBPGR), Regional Station, Srinagar-190 005, Jammu & Kashmir, India
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Milind Ratnaparkhe
Indian Institute of Soybean Research, Indore-452 001, Madhya Pradesh, India
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Jagadish Rane
National Institute of Abiotic Stress Management
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Rajeev Varshney
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
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Aardra Kachroo
Univ Kentucky
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Abstract

FAD3 play important roles in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of FAD3 in drought, salinity and heat stress tolerance is lacking in soybean. The present study assessed the functional role of fatty acid desaturase 3 to abiotic stress responses in soybean. We used Bean Pod Mottle Virus -based vector to alter expression of Glycine max omega-3 fatty acid desaturase . Higher levels of recombinant BPMV-GmFAD3 transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3 in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. FAD3 overexpressing plants showed higher levels of chlorophyll content, leaf SPAD value, relative water content, chlorophyll fluorescence, transpiration rate, carbon assimilation rate, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from current study revealed that GmFAD3 overexpressing soybean plants exhibited drought and salinity stress tolerance although tolerance to heat stress was reduced. On the other hand, soybean plants silenced for GmFAD3 exhibited tolerance to heat stress, but were vulnerable to drought and salinity stress