Characterization of Four Novel H5N6 Avian Influenza Viruses with the
Internal Genes from H5N1 and H9N2 Viruses and Experimental Challenge of
Chickens Vaccinated with Current Commercially Available H5 Vaccines
- Peng Chen,
- Pengwei Zhao,
- Jun Chu,
- Junda Zhu,
- Qiuchen Li,
- Haiyuan Zhao,
- Yujie Li,
- Lingxiang Xin,
- Xiaoyue Yang,
- Shijie Xie,
- Changdong Zhu,
- wenbao Qi,
- Guanlong Xu,
- Jinxiang Li
Peng Chen
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
Corresponding Author:[email protected]
Author ProfilePengwei Zhao
Department of Biochemistry and Molecular Biology, School of Medicine, Zhejiang University
Author ProfileJunda Zhu
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
Author ProfileQiuchen Li
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
Author ProfileJinxiang Li
National Agricultural Science and Technology Center
Author ProfileAbstract
Since 2014, highly pathogenic avian influenza H5N6 viruses have been
responsible for outbreaks in poultry. In this study, four H5N6 virus
strains were isolated from fecal samples of sick white ducks and dead
chickens in Shandong in 2019. These H5N6 viruses were triple-reassortant
viruses that have not been previously characterized. Their HA genes were
derived from the H5 viruses and were closely related to the vaccine
strain Re-11. Their NA genes all fell into the N6-like lineage and the
internal gene were derived from H5N1 and H9N2 viruses. They all showed
high pathogenicity in mice and caused lethal infection with high rates
of transmission in chickens. Moreover, the SPF chickens inoculated with
the current used vaccine in China were completely protected from these
four H5N6 viruses. Our study indicated the necessity of continued
surveillance for H5 IAV and the importance of timely update of vaccine
strains in poultry industry.02 Oct 2020Submitted to Transboundary and Emerging Diseases 18 Nov 2020Submission Checks Completed
18 Nov 2020Assigned to Editor
29 Nov 2020Reviewer(s) Assigned
15 Dec 2020Review(s) Completed, Editorial Evaluation Pending
15 Dec 2020Editorial Decision: Revise Minor
23 Dec 20201st Revision Received
11 Mar 2021Assigned to Editor
11 Mar 2021Submission Checks Completed
12 Mar 2021Reviewer(s) Assigned
22 Mar 2021Review(s) Completed, Editorial Evaluation Pending
23 Mar 2021Editorial Decision: Revise Minor
08 Apr 20212nd Revision Received
08 Apr 2021Submission Checks Completed
08 Apr 2021Assigned to Editor
08 Apr 2021Reviewer(s) Assigned
09 Apr 2021Review(s) Completed, Editorial Evaluation Pending
13 Apr 2021Editorial Decision: Accept
