loading page

High-quality genome assembly, annotation and evolutionary analysis of the mungbean (Vigna radiata) genome
  • +8
  • Qiang Yan,
  • Qiong Wang,
  • Cheng Xuzhen,
  • Lixia Wang,
  • Prakit Somta,
  • Chenchen Xue,
  • Jingbin Chen,
  • Ranran Wu,
  • Yun Lin,
  • Xingxing Yuan,
  • Xin Chen
Qiang Yan
Jiangsu Academy of Agricultural Sciences
Author Profile
Qiong Wang
Jiangsu Academy of Agricultural Sciences
Author Profile
Cheng Xuzhen
Chinese Academy of Agricultural Sciences
Author Profile
Lixia Wang
Chinese Academy of Agricultural Sciences
Author Profile
Prakit Somta
Kasetsart University Kamphaeng Saen Campus
Author Profile
Chenchen Xue
Jiangsu Academy of Agricultural Sciences
Author Profile
Jingbin Chen
Jiangsu Academy of Agricultural Sciences
Author Profile
Ranran Wu
Jiangsu Academy of Agricultural Sciences
Author Profile
Yun Lin
Jiangsu Academy of Agricultural Sciences
Author Profile
Xingxing Yuan
Jiangsu Academy of Agricultural Sciences
Author Profile
Xin Chen
Jiangsu Academy of Agricultural Sciences
Author Profile

Abstract

Mungbean (Vigna radiata [L.]) is an important economic crop grown in South, and East Asia. The low contiguity of the current assembly of V. radiata genome has limited its application. Here, we report a high-quality chromosome-scale assembled genome of V. radiata to facilitate the investigation of its genome characteristics and evolution. By combination of Nanopore long reads, Illumina short reads and Hi-C data, we generated a high-quality genome assembly of V. radiata, with 473.67 megabases assembled into 11 chromosomes with contig N50 and scaffold N50 of 11.3 and 42.4 megabases, respectively. A total of 52.8% of the genome was annotated as repetitive sequences, among which LTRs (long terminal repeats) were predominant (33.9%). The genome of V. radiata was predicted to contain 33,924 genes, 32,470 (95.7%) of which could be functionally annotated. Evolutionary analysis revealed an estimated divergence time of V. radiata from its close relative V. angularis of ~11.66 million years ago. In addition, 277 V. radiata specific gene families, 18 positively selected genes were detected and functionally annotated. This high-quality mungbean genome will provide valuable resources for further genetic analysis and crop improvement of mungbean and other legume species.