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Circulating CD15+LOX-1+ PMN-MDSCs are a potential biomarker for the early diagnosis of non-small cell lung cancer
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  • Xinyu Tian,
  • Ting Wang,
  • Qisi Zheng,
  • Yue Tao,
  • Lei Dai,
  • Han Shen
Xinyu Tian
Nanjing Drum Tower Hospital

Corresponding Author:[email protected]

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Ting Wang
Jiangsu Cancer Hospital
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Qisi Zheng
Nanjing Drum Tower Hospital
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Yue Tao
Nanjing Drum Tower Hospital
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Lei Dai
Nanjing Drum Tower Hospital
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Han Shen
Nanjing Drum Tower Hospital
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Abstract

Aims: Non-small-cell lung cancer (NSCLC) is the most common clinical lung cancer. Polymorphonuclear-myeloid derived suppressor cells (PMN-MDSCs), which are the major population of MDSCs, are involved in NSCLC progression. Recently, it was found that lectin-type oxidized LDL receptor 1 (LOX-1) could identify humsn PMN-MDSCs. However, the role of CD15+LOX-1+ PMN-MDSCs in NSCLC early diagnosis has not been revealed. Here, we tried to confirm the application of the newly-identified CD15+LOX-1+ PMN-MDSCs in the early diagnosis of NSCLC. Methods: Flow cytometry (FCM) was used to detect the proportion of CD15+LOX-1+ PMN-MDSCs in the peripheral blood (PB) of healthy controls (HC) and NSCLC patients. The correlation of CD15+LOX-1+ PMN-MDSC frequency with levels of cytokeratin 19-fragments (CYFRA21-1), carcinoembryonic antigen (CEA), and carbohydrate antigen 125 (CA125) was analyzed. Receiver operating characteristic (ROC) curve was used to estimate the diagnostic efficacy of CD15+LOX-1+ PMN-MDSCs for NSCLC. Additionally, the association of CD15+LOX-1+ PMN-MDSC frequency with NSCLC prognosis/recurrence after surgery was explored. Results: The proportion of CD15+LOX-1+ PMN-MDSCs increased in PB of NSCLC patients. CD15+LOX-1+ PMN-MDSC proportion was positively correlated with levels of CEA and CYFRA21-1. The area under the ROC curve (AUC) of PMN-MDSC percentage was higher than CYFRA21-1, CEA and CA125. The proportion of CD15+LOX-1+ PMN-MDSCs decreased in patients after surgery. The frequency of CD15+LOX-1+ PMN-MDSCs was lower in NSCLC patients without recurrence compared to those with recurrence after surgery. Conclusions: Circulating CD15+LOX-1+ PMN-MDSCs are a potential diagnostic marker for NSCLC, and are associated with NSCLC prognosis and recurrence after surgery.
29 Nov 2020Submitted to International Journal of Clinical Practice
29 Nov 2020Submission Checks Completed
29 Nov 2020Assigned to Editor
01 Dec 2020Reviewer(s) Assigned
09 Mar 2021Review(s) Completed, Editorial Evaluation Pending
29 Mar 20211st Revision Received
01 Apr 2021Submission Checks Completed
01 Apr 2021Assigned to Editor
08 Apr 2021Review(s) Completed, Editorial Evaluation Pending
03 May 2021Editorial Decision: Accept