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Potassium limitation promotes the Sweetgum-Clitopilus symbiosis
  • +9
  • long peng,
  • Xiaoliang Shan,
  • Yuzhan Yang,
  • Yuchen Wang,
  • Irina Druzhinina,
  • Xueyu Pan,
  • Wei Jin,
  • Xinghua He,
  • Xinyu Wang,
  • Xiaoguo Zhang,
  • Francis Martin,
  • Zhilin Yuan
long peng
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Xiaoliang Shan
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Yuzhan Yang
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Yuchen Wang
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Irina Druzhinina
Nanjing Agricultural University
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Xueyu Pan
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Wei Jin
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Xinghua He
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Xinyu Wang
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Xiaoguo Zhang
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Francis Martin
INRA Nancy
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Zhilin Yuan
Research Institute of Subtropical Forestry Chinese Academy of Forestry
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Abstract

Several species of soil free-living saprotrophs can sometimes establish biotrophic symbiosis with plants, but the basic biology of this association remains largely unknown. Here, we investigate the symbiotic interaction between a common soil saprotroph, Clitopilus hobsonii (Agaricomycetes), and the American sweetgum (Liquidambar styraciflua). Notably, the colonized root cortical cells contain numerous microsclerotia-like structures. Fungal colonization led to increased plant growth and facilitated potassium uptake, particularly under potassium limitation (0.05 mM K+). The expression of plant genes related to potassium uptake is not altered during symbiosis, whereas the transcripts of three fungal genes encoding ACU, HAK, and SKC involved in K+ nutrition is found in colonized roots. We confirmed the K+ influx activities by expressing the ChACU and ChSKC genes into a yeast K+-uptake-defective mutant. Upregulation of the ChACU under 0.05 mM K+ and no K+ conditions was demonstrated in planta and in vitro compared to normal condition (5 mM K+). In addition, colonized plants displayed a larger accumulation of soluble sugars under 0.05 mM K+. The present study highlights that potassium limitation promotes this novel tree-fungus symbiosis mainly through a reciprocal transfer of additional carbon and potassium to both partners, and the role of dual soil saprotroph/symbiotroph in tree nutrition.

Peer review status:ACCEPTED

06 Dec 2020Submitted to Plant, Cell & Environment
07 Dec 2020Submission Checks Completed
07 Dec 2020Assigned to Editor
12 Dec 2020Reviewer(s) Assigned
26 Dec 2020Review(s) Completed, Editorial Evaluation Pending
31 Dec 2020Editorial Decision: Revise Minor
19 Jan 20211st Revision Received
22 Jan 2021Assigned to Editor
22 Jan 2021Submission Checks Completed
27 Jan 2021Review(s) Completed, Editorial Evaluation Pending
30 Jan 2021Editorial Decision: Revise Minor
03 Feb 20212nd Revision Received
04 Feb 2021Assigned to Editor
04 Feb 2021Submission Checks Completed
12 Feb 2021Review(s) Completed, Editorial Evaluation Pending
12 Feb 2021Editorial Decision: Revise Minor
15 Feb 20213rd Revision Received
15 Feb 2021Submission Checks Completed
15 Feb 2021Assigned to Editor
21 Feb 2021Review(s) Completed, Editorial Evaluation Pending
21 Feb 2021Editorial Decision: Revise Minor
22 Feb 20214th Revision Received
22 Feb 2021Assigned to Editor
22 Feb 2021Submission Checks Completed
24 Feb 2021Review(s) Completed, Editorial Evaluation Pending
12 Mar 2021Editorial Decision: Revise Minor
15 Mar 20215th Revision Received
16 Mar 2021Submission Checks Completed
16 Mar 2021Assigned to Editor
18 Mar 2021Review(s) Completed, Editorial Evaluation Pending
18 Mar 2021Editorial Decision: Accept