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Insights into algae evolution for adapting to blue-green light garnered from the Isochrysis galbana genome
  • +17
  • Duo Chen,
  • Xue Yuan,
  • Xuehai Zheng,
  • Jingping Fang,
  • Gang Lin,
  • Jiannan Chen,
  • Daliang Li,
  • Li Li,
  • Wejin He,
  • Luqiang Huang,
  • Zhen Huang,
  • Rongmao Li,
  • Yajuan Fu,
  • Wenfang Fan,
  • Limin Liang,
  • Nan Chen,
  • Chentao Lin,
  • Jinmao Zhu,
  • Youqiang Chen,
  • Ting Xue
Duo Chen
Fujian Normal University
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Xue Yuan
Fujian Normal University
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Xuehai Zheng
Fujian Normal University
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Jingping Fang
Fujian Normal University
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Gang Lin
Fujian Normal University
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Jiannan Chen
Fujian Normal University
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Daliang Li
Fujian Normal University
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Li Li
Fujian Normal University
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Wejin He
Fujian Normal University
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Luqiang Huang
Fujian Normal University
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Zhen Huang
Fujian Normal University
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Rongmao Li
Fujian fishery resources monitoring center
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Yajuan Fu
Fujian Normal University
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Wenfang Fan
Fujian Normal University
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Limin Liang
Fujian Normal University
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Nan Chen
College of Fine Arts, Fujian Normal University,
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Chentao Lin
Fujian Normal University
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Jinmao Zhu
Fujian Normal University
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Youqiang Chen
Fujian Normal University
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Ting Xue
Fujian Normal University
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Abstract

Isochrysis galbana is an important producer in the aquatic ecosystem because of its rich fucoxanthin content and fast growth. However, little is known about its evolutionary adaptation to live in a specific, complex and harsh environment. We report a high-quality genome sequence of I. galbana LG007, which has a 92.73 Mb genome size, a contig N50 of 6.99 Mb and 14,900 protein-coding genes. Phylogenomic inferences confirmed the monophyly of Haptophyta, showing I. galbana is a sister to E. huxleyi and C. tobinii. Evolutionary analysis revealed an estimated divergence of I. galbana from its close relative E. huxleyi ~133 million years ago, and I. galbana underwent one round of whole-genome duplication. Genes related to environmental adaptation and metabolic regulation in I. galbana were relatively conserved, but the basic transcriptional regulation toolkit for terrestrial plant development has been contracted or was not detected. The domain identification of one novel fucoxanthin biosynthesis gene that encodes diadinoxanthin-fucoxanthin hydroxylase (DFH) was investigated. Comparative genomic analysis revealed that I. galbana acquired several transcription factors specific to the control of pigment accumulation and production of LHCX2 to harvest blue-green light, which facilitates adaptation to the underwater environment. These findings provide new insights into the genomic characteristics of I. galbana and algae evolution for adaptation to blue-green light underwater.

Peer review status:UNDER REVIEW

13 Nov 2020Submitted to Molecular Ecology Resources
18 Dec 2020Assigned to Editor
18 Dec 2020Submission Checks Completed
24 Dec 2020Reviewer(s) Assigned
10 Jan 2021Review(s) Completed, Editorial Evaluation Pending