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Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with silver nanoparticles synthesized using Eucalyptus citriodora leaf extract
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  • Supakit Paosen,
  • Sakkarin Lethongkam,
  • Suttiwan Wunnoo,
  • Nussana Lehman,
  • Ekwipoo Kalkornsurapranee,
  • Supayang Voravuthikunchai
Supakit Paosen
Prince of Songkla University
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Sakkarin Lethongkam
Prince of Songkla University
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Suttiwan Wunnoo
Prince of Songkla University
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Nussana Lehman
Prince of Songkla University
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Ekwipoo Kalkornsurapranee
Prince of Songkla University
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Supayang Voravuthikunchai
Prince of Songkla University
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Abstract

Failure in the prevention of cross-transmission from contaminated gloves has been recognized as an important factor that contributes to the spread of several healthcare-associated infections. Ex situ coating process with silver nanoparticles (AgNPs) using Eucalyptus citriodora ethanolic leaf extract as reducing and capping agents to coat glove surfaces has been developed to prevent this mode of transmission. Elemental analysis of coated gloves showed 24.8 Wt% silver densely adhere on the glove surface. The coated gloves fully eradicated important hospital-acquired pathogens including Gram-positive bacteria, Gram-negative bacteria, and yeasts within 1 h. The coated gloves showed significant reduction, an average of 5 logs when tested against all standard strains and most clinical isolates (p < 0.01). Following prolonged exposure, the coating significantly reduced the numbers of most adhered pathogenic species, compared with uncoated gloves (p < 0.0001), which was observed by fluorescence microscopy. Scanning electron microscopy further confirmed that AgNPs coated-gloves reduced microbial adhesion of mixed-species biofilms, compared with uncoated gloves. A series of contamination and transmission assays demonstrated no transmission of viable organisms. Biocompatibility analysis confirmed high cell viability of HaCaT and L929 cells at all concentrations of AgNPs tested. The coated gloves were non-toxic with direct contact with L929 cells.

Peer review status:ACCEPTED

19 Jan 2021Submitted to Biotechnology Journal
19 Jan 2021Submission Checks Completed
19 Jan 2021Assigned to Editor
22 Jan 2021Reviewer(s) Assigned
22 Apr 2021Editorial Decision: Revise Minor
05 May 20211st Revision Received
07 May 2021Submission Checks Completed
07 May 2021Assigned to Editor
07 May 2021Reviewer(s) Assigned
27 May 2021Editorial Decision: Revise Minor
02 Jun 20212nd Revision Received
03 Jun 2021Submission Checks Completed
03 Jun 2021Assigned to Editor
03 Jun 2021Reviewer(s) Assigned
03 Jun 2021Editorial Decision: Accept