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Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan
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  • Miwako Kasahara-Kamiie,
  • Mitsuo Kagawa,
  • Mai Shiokawa,
  • Fujiko Sunaga,
  • Yuka Fukase,
  • Naoyuki Aihara,
  • Takanori Shiga,
  • Junichi Kamiie,
  • Hiroshi Aoki,
  • Makato Nagai
Miwako Kasahara-Kamiie
Azabu University
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Mitsuo Kagawa
Kagawa Livestock Hospital
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Mai Shiokawa
Nippon Veterinary and Life Science University
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Fujiko Sunaga
Azabu University
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Yuka Fukase
Azabu University
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Naoyuki Aihara
Azabu University
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Takanori Shiga
Azabu University
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Junichi Kamiie
Azabu University
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Hiroshi Aoki
Nippon Veterinary and Life Science University
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Makato Nagai
Azabu University
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Abstract

Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered porcine virus that has been found in the Americas, Europe, and Asia; however, no report of APPV in Japan has been published. We identified an APPV in the central nervous system of Japanese piglets with CT, and firstly determined and analyzed the complete genome sequence. Phylogenetic analysis using the complete genome nucleotide sequence of the Japanese APPV, named Anna/2020, and those of APPVs from the NCBI database showed that APPVs were divided into three genotypes (genotypes 1 to 3), and that Anna/2020 clustered with the genotype 3 APPV strains, but distantly branched from these strains. Pairwise complete coding region nucleotide sequence comparisons revealed that there was 94.0% to 99.7% sequence identity among the genotype 3 strains, while Anna/2020 showed 87.0% to 89.3% identity to those genotype 3 strains, suggesting that Anna/2020 represents a novel APPV lineage within genotype 3. Retrospective examinations using RT-PCR revealed one genotype 1 and two novel genotype 3 APPVs from pigs without CT, and that novel genotype 3 APPVs have been prevalent in Japan since at least 2007.

Peer review status:ACCEPTED

19 Jan 2021Submitted to Transboundary and Emerging Diseases
19 Jan 2021Submission Checks Completed
19 Jan 2021Assigned to Editor
24 Jan 2021Reviewer(s) Assigned
24 Mar 2021Review(s) Completed, Editorial Evaluation Pending
29 Apr 2021Editorial Decision: Revise Major
06 May 20211st Revision Received
06 May 2021Submission Checks Completed
06 May 2021Assigned to Editor
07 May 2021Review(s) Completed, Editorial Evaluation Pending
07 May 2021Editorial Decision: Accept