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Haplotypes within tandemly duplicated candidate genes at BnaA9.MRP5 modulate phytate concentration in canola (Brassica napus L.)
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  • Haijiang Liu,
  • xiaojuan Li,
  • Qianwen Zhang,
  • pan yuan,
  • Lei Liu,
  • Graham King,
  • Guangda Ding,
  • Sheliang Wang,
  • Hongmei Cai,
  • Chuang Wang,
  • Fangsen XU,
  • Lei Shi
Haijiang Liu
Huazhong Agricultural University
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xiaojuan Li
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Qianwen Zhang
Shanghai Jiao Tong University School of Agriculture and Biology
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pan yuan
Huazhong Agricultural University
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Lei Liu
Southern Cross University
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Graham King
Southern Cross University
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Guangda Ding
Huazhong Agricultural University
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Sheliang Wang
Huazhong Agricultural University
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Hongmei Cai
Huazhong Agricultural University
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Chuang Wang
Huazhong Agricultural University
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Fangsen XU
Huazhong Agricultural University
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Lei Shi
Huazhong Agricultural University
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Abstract

Phytate is the storage form of phosphorus in angiosperm seeds and plays vitally important roles during seed development. However, in crop plants phytate decreases bioavailability of seed-sourced mineral elements for humans, livestock and poultry, and contributes to phosphate-related water pollution. However, there is little knowledge about this trait in oilseed rape B. napus (oilseed rape). Here, a panel of 505 diverse B. napus accessions was screened in a genome-wide association study (GWAS) using 3.28 x 106 single nucleotide polymorphisms (SNPs). This identified 119 SNPs significantly associated with phytate concentration (PA_Conc) and phytate content (PA_Cont) and six candidate genes were identified. Of these, BnaA9.MRP5 represented the candidate gene for the significant SNP chrA09_5198034 (27kb) for both PA_Cont and PA_Conc. Transcription of BnaA9.MRP5 in a low -phytate variety (LPA20) was significantly elevated compared with a high -phytate variety (HPA972). Association and haplotype analysis indicated that inbred lines carrying specific SNP haplotypes within BnaA9.MRP5 were associated with high- and low-phytate phenotypes. No significant differences in seed germination and seed yield were detected between low and high phytate cultivars examined. Candidate genes, favorable haplotypes and the low phytate varieties identified in this study will be useful for low-phytate breeding of B. napus.