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Investigation the levels of endotoxin and 8-OHdG in sera of patients with H.pyloripositive peptic ulcer
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  • Nihayet Bayraktar,
  • İslim Guler,
  • Ismail Koyuncu,
  • Mehmet Bayraktar
Nihayet Bayraktar
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İslim Guler
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Ismail Koyuncu
Harran University
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Mehmet Bayraktar
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Abstract

Background and aim: Peptic ulcer is considered an important public health problem and generally associated with complicated conditions such as bleeding and perforation. The aim of this study is to reflect the rate of oxidative damage in the body among patients with H. pylori positive peptic ulcer by measuring serum 8-hydroxy-2p-deoxyguanosine (8-OHdG) and its association with the level of bacterial endotoxin. Methods Patients applied to Harran University Gastroenterology Outpatient Clinic with dyspeptic complaints were enrolled in this study. According to gastrointestinal endoscopy findings, 43 patients with H.pylori positive peptiv ulcer patients and 43 healthy volunteers were included in this study. H.pylori diagnosis was detemined by H.pylori urea breath and stool antigen tests. Serum 8-OHdG and endotoxins were measued by ELISA. Results A total of 43 patients with peptic ulcer (13 females 30 males), 43 healthy individuals (16 females 27 males) ages (18- 70) years in the study. In biopsies taken endoscopically; Hp severity was mild in 19 patients (43.9%), moderate in 21 patients (48.5%), and severe in 3 patients (7.6%). 8-OHdG which has the potential to mark DNA damage level in serum samples of patients with H.pylori positive peptic ulcer, was compared with the healthy and patient group. It was observed that there was a statistically significant difference (p <0.01). In addition, a weak correlation was found between OHdG and endotoxin. Conclusion: Reactive oxygen species (ROS) produced due to increased endotoxin as a result of H. pylıori infection can attack nucleic acid in infected cells resulting in an increased in serum 8-OHdG level. H.pylori and its endotoxin had a significant in peptic ulcer pathogensis.

Peer review status:ACCEPTED

17 Jan 2021Submitted to International Journal of Clinical Practice
22 Jan 2021Submission Checks Completed
22 Jan 2021Assigned to Editor
01 Feb 2021Reviewer(s) Assigned
23 Mar 2021Review(s) Completed, Editorial Evaluation Pending
19 May 20211st Revision Received
21 May 2021Submission Checks Completed
21 May 2021Assigned to Editor
21 May 2021Review(s) Completed, Editorial Evaluation Pending
25 May 2021Reviewer(s) Assigned
10 Jun 2021Editorial Decision: Accept