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CD5 expressing CD8+ T cell subsets differ between children with Type 1 Diabetes and controls
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  • Josefine Wadenpohl,
  • Julia Seyfarth,
  • Paul Hehenkamp,
  • Maximilian Hoffmann,
  • Sebastian Kummer,
  • Christina Reinauer,
  • Carsten Döing,
  • Katharina Förtsch,
  • Ertan Mayatepek,
  • Thomas Meissner,
  • Marc Jacobsen
Josefine Wadenpohl
Heinrich-Heine-Universitat Dusseldorf
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Julia Seyfarth
Heinrich-Heine-Universitat Dusseldorf
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Paul Hehenkamp
Heinrich-Heine-Universitat Dusseldorf
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Maximilian Hoffmann
Heinrich-Heine-Universitat Dusseldorf
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Sebastian Kummer
Heinrich-Heine-Universitat Dusseldorf
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Christina Reinauer
Heinrich-Heine-Universitat Dusseldorf
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Carsten Döing
Heinrich-Heine-Universitat Dusseldorf
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Katharina Förtsch
Heinrich-Heine-Universitat Dusseldorf
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Ertan Mayatepek
Heinrich-Heine-Universitat Dusseldorf
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Thomas Meissner
Heinrich-Heine-Universitat Dusseldorf
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Marc Jacobsen
Heinrich-Heine-Universitat Dusseldorf
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Abstract

Different lymphocyte subsets are involved in autoimmune pathogenesis of Type 1 Diabetes (T1D). Previous studies suggested a role of CD5 expressing T and B cells including rare unconventional lymphocytes with combined T- and B-cell features (DE cells). We performed algorithm-supported multi-parameter flow cytometry and quantitative PCR to investigate immune cell subsets and DE cells in children with T1D (n=20) and matched controls (n=20). Comparisons of conventional immune cells detected increased proportions of CD3+ T cells in T1D patients whereas CD19+ B cell proportions were comparable to controls. Self-organizing maps for flow cytometry analyses (FlowSOM) showed highly similar CD5 expressing B-cell subsets and no differences for DE cells were detected between the study groups by flow cytometry or specific quantitative PCR. Notably, differences in CD8 positive T cells were indicated by FlowSOM and similarity-based tSNE analyses. Study group comparison confirmed significantly reduced CD8+ T-cell proportions with moderate or low CD5 expression in T1D patients. Finally, In vitro experiments showed stable CD5 expression differences of CD8+ T cells after T-cell activation, cytokine stimulation and culture. We observed differences of T-cell co-receptor CD5 expression in T1D patients with potential relevance for immune regulation of CD8+ T-cell activation.