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A hunt for OM45 synthetic petite interactions in Saccharomyces cerevisiae reveals a role for Miro GTPase Gem1 in cristae structure maintenance
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  • Antonina Shvetsova,
  • Ali Masud,
  • Laura Schneider,
  • Geoffray Monteuuis,
  • Ulrich Bergmann,
  • Ilkka Miinalainen,
  • J. Kalervo Hiltunen,
  • Alexander Kastaniotis
Antonina Shvetsova
University of Oulu
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Ali Masud
University of Oulu
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Laura Schneider
University of Oulu
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Geoffray Monteuuis
University of Oulu
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Ulrich Bergmann
University of Oulu
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Ilkka Miinalainen
University of Oulu
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J. Kalervo Hiltunen
University of Oulu
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Alexander Kastaniotis
University of Oulu
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Abstract

Om45 is a major protein of the yeast outer mitochondrial membrane under respiratory conditions. However, the cellular role of the protein has remained obscure. Previously, deletion mutant phenotypes have not been found, and clear amino acid sequence similarities that would allow inferring its functional role are not available. In this work, we describe synthetic petite mutants of UGO1 and GEM1 that depend on the presence of OM45 for respiratory growth, as well as the identification of several multicopy suppressors of the synthetic petite phenotypes. In the analysis of our mutants, we demonstrate that Om45 and Gem1 have a collaborative role in the maintenance of mitochondrial morphology, cristae structure, and mitochondrial DNA maintenance. A group of multicopy suppressors rescuing the synthetic lethal phenotypes of the mutants on non-fermentable carbon sources additionally supports this result. Our results imply that the synthetic petite phenotypes we observed are due to the disturbance of the inner mitochondrial membrane and point to this mitochondrial sub-compartment as the main target of action of Om45, Ugo1, and the yeast Miro GTPase Gem1.

Peer review status:IN REVISION

23 Mar 2021Submitted to MicrobiologyOpen
24 Mar 2021Assigned to Editor
24 Mar 2021Submission Checks Completed
24 Mar 2021Reviewer(s) Assigned
07 Apr 2021Review(s) Completed, Editorial Evaluation Pending
07 Apr 2021Editorial Decision: Revise Major