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Preparation of Komagataeibacter xylinus inoculum for bacterial cellulose biosynthesis using magnetically assisted external-loop airlift bioreactor
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  • Anna Żywicka,
  • Daria Ciecholewska-Juśko,
  • Radosław Drozd,
  • Rafał Rakoczy,
  • Maciej Konopacki,
  • Marian Kordas,
  • Adam Junka,
  • Paweł Migdał,
  • Karol Fijałkowski
Anna Żywicka
West Pomeranian University of Technology
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Daria Ciecholewska-Juśko
West Pomeranian University of Technology
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Radosław Drozd
West Pomeranian University of Technology
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Rafał Rakoczy
West Pomeranian University of Technology
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Maciej Konopacki
West Pomeranian University of Technology
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Marian Kordas
West Pomeranian University of Technology
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Adam Junka
Wroclaw Medical University
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Paweł Migdał
Wroclaw University of Environmental and Life Sciences
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Karol Fijałkowski
West Pomeranian University of Technology
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Abstract

The aim of this study was to demonstrate the applicability of a novel magnetically-assisted external-loop airlift bioreactor (EL-ALB), equipped with RMF generators for the preparation of Komagataeibacter xylinus inoculum during three-cycle repeated fed-batch cultures, further used for bacterial cellulose (BC) production. The fermentation carried out in the RMF-assisted EL-ALB allowed to obtain an inoculum of more than 200x higher cellular density compared to classical methods of inoculum preparation. The inoculum obtained in the RMF-assisted EL-ALB was characterized by a high and stable metabolic activity during repeated batch fermentation process. The application of the RMF-assisted EL-ALB for K. xylinus inoculum production did not induce the formation of cellulose-deficient mutants. It was also confirmed that the ability of K. xylinus to produce BC was at the same level (7.26 g/L of dry mass), regardless of inoculum age. Additionally, the BC obtained from the inoculum produced in the RMF-assisted EL-ALB was characterized by reproducible mechanical strength, nanostructure and total crystallinity index. The results obtained in this study may find multiple applications in any biotechnological processes requiring a high-quality bacterial inoculum.