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Unravelling the plant diversity of the Amazonian canga through DNA barcoding
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  • Santelmo Vasconcelos,
  • Gisele Nunes,
  • Mariana Dias,
  • Jamily Lorena,
  • Renato Oliveira,
  • Talvâne Lima,
  • Eder Pires,
  • Rafael Valadares,
  • Ronnie Alves,
  • Maurício Watanabe,
  • Daniela Zappi,
  • Alice Hiura,
  • Mayara Pastore,
  • Liziane Vasconcelos,
  • Nara Mota,
  • Pedro Viana,
  • André Gil,
  • Andre Simões,
  • Vera Imperatriz-Fonseca,
  • Raymond Harley,
  • Ana Giulietti,
  • Guilherme Oliveira
Santelmo Vasconcelos
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Gisele Nunes
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Mariana Dias
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Jamily Lorena
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Renato Oliveira
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Talvâne Lima
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Eder Pires
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Rafael Valadares
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Ronnie Alves
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Maurício Watanabe
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Daniela Zappi
Universidade de Brasilia
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Alice Hiura
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Mayara Pastore
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Liziane Vasconcelos
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Nara Mota
Museu Paraense Emilio Goeldi Campus de Pesquisa
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Pedro Viana
Museu Paraense Emilio Goeldi Campus de Pesquisa
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André Gil
Museu Paraense Emilio Goeldi Campus de Pesquisa
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Andre Simões
Universidade Estadual de Campinas - Campus Cidade Universitaria Zeferino Vaz
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Vera Imperatriz-Fonseca
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Raymond Harley
Royal Botanic Gardens Kew
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Ana Giulietti
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Guilherme Oliveira
Instituto Tecnológico Vale Desenvolvimento Sustentável
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Abstract

The canga of the Serra dos Carajás, in Eastern Amazon, is home to a unique open plant community, harbouring several endemic and rare species. Although a complete flora survey has been recently published, scarce to no genetic information is available for most plant species of the ironstone outcrops of the Serra dos Carajás. In this scenario, DNA barcoding appears as a fast and effective approach to assess the genetic diversity of the Serra dos Carajás flora, considering the growing need for robust biodiversity conservation planning in such an area with industrial mining activities. Thus, after testing eight different DNA barcode markers (matK, rbcL, rpoB, rpoC1, atpF-atpH, psbK-psbI, trnH-psbA and ITS2), we chose rbcL and ITS2 as the most suitable markers for a broad application in the regional flora. Here we describe DNA barcodes for 1,130 specimens of 538 species, 323 genera and 115 families of vascular plants, with a total of 344 species being barcoded for the first time. In addition, we assessed the potential of using DNA metabarcoding of bulk samples for surveying plant diversity in the canga. Upon achieving the first comprehensive DNA barcoding effort directed to a complete flora in the Brazilian Amazon, we discuss the relevance of our results to guide future conservation measures in the Serra dos Carajás.

Peer review status:UNDER REVIEW

09 Apr 2021Submitted to Molecular Ecology Resources
13 Apr 2021Assigned to Editor
13 Apr 2021Submission Checks Completed
19 Apr 2021Reviewer(s) Assigned
20 May 2021Review(s) Completed, Editorial Evaluation Pending