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Relationship of E-cadherin, Beta-catenin, N-cadherin, ZEB1 and αSMA as Epithelial Mesenchymal Transition markers with prognostic factors in early and advanced stage laryngeal squamous cell carcinomas
  • +2
  • ULKU KUCUK,
  • Sumeyye EKMEKCI,
  • Canan Kelten Talu,
  • Yeliz PEKCEVIK,
  • Ibrahim CUKUROVA
ULKU KUCUK
Republic of Turkey Ministry of Health Izmir Provincial Health Directorate Izmir University of Health Sciences Tepecik Training and Research Hospital
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Sumeyye EKMEKCI
Republic of Turkey Ministry of Health Izmir Provincial Health Directorate Izmir University of Health Sciences Tepecik Training and Research Hospital
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Canan Kelten Talu
Republic of Turkey Ministry of Health Izmir Provincial Health Directorate Izmir University of Health Sciences Tepecik Training and Research Hospital
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Yeliz PEKCEVIK
Republic of Turkey Ministry of Health Izmir Provincial Health Directorate Izmir University of Health Sciences Tepecik Training and Research Hospital
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Ibrahim CUKUROVA
Republic of Turkey Ministry of Health Izmir Provincial Health Directorate Izmir University of Health Sciences Tepecik Training and Research Hospital
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Abstract

Objective: To investigate the relationship between E-cadherin, Beta-catenin, N-cadherin, ZEB1 and αSMA as eithelial mesenchymal transformation markers with tumor stage, lymph node metastasis (LNM) and overall survival (OS) in laryngeal squamous cell carcinomas (LSCC). Material and method: A total of 100 cases diagnosed with LSCC in our hospital between 2013-2020 were included in the study. Data about lymphovascular invasion (LVI), perineural invasion (PNI), necrosis and LNM were recorded by evaluating hematoxylin- eosin stained slides. Markers of E-cadherin, beta-catenin, N-cadherin, ZEB1 and αSMA were applied to the sections prepared from paraffin blocks of tumor samples. Results: Ninety-five male and five female patients were included in the study, and 38 of them exited. The average OS time of the cases was 35.8 months. A significant relationship was observed between OS with advanced tumor stage, presence of LNM and PNI. A significant relationship was found between increased tumor Zeb1 expression and advanced tumor stage. In univariate and multivariate analyses, a significant negative relationship with OS, and increased Zeb1 expression in tumor and tumor stroma was seen. Any relationship was not observed between E-cadherin, beta-catenin, N-cadherin and αSMA and OS. , Conclusion Among the EMT markers we evaluated in our study, it was seen that Zeb1, which is an EMT transcription factor, is associated with tumor stage, LNM, and OS. Remarkably, Zeb1 expression observed in tumor stroma was also significant for OS. Any similar data reported for LSCCs have not been encountered in the literature, and it was thought that it would be appropriate to support our findings with further studies to be performed on this subject.