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Optimization of E. coli Tip-Sonication for High-Yield Cell Free Extract using Finite Element Modeling
  • Md Sakib Ferdous,
  • Jared Dopp,
  • Nigel Reuel
Md Sakib Ferdous
Iowa State University of Science and Technology
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Jared Dopp
Iowa State University of Science and Technology
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Nigel Reuel
Iowa State University of Science and Technology
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Abstract

Optimal tip sonication settings, namely tip position, input power, and pulse durations, are necessary for temperature sensitive procedures like preparation of viable cell extract. In this paper, the optimum tip immersion depth (20-30% height below the liquid surface) is estimated which ensures maximum mixing thereby enhancing thermal dissipation of local cavitation hotspots. A finite element (FE) heat transfer model is presented, validated experimentally with (R2 > 97%) and used to observe the effect of temperature rise on cell extract performance of E. coli BL21 DE3 star strain and estimate the temperature threshold. Relative yields in the top 10% are observed for solution temperatures maintained below 32°C; this reduces below 50% relative yield at temperatures above 47°C. A generalized workflow for direct simulation using the COMSOL code as well as master plots for estimation of sonication parameters (power input and pulse settings) is also presented.

Peer review status:UNDER REVIEW

27 Apr 2021Submitted to AIChE Journal
02 May 2021Assigned to Editor
02 May 2021Submission Checks Completed
06 May 2021Reviewer(s) Assigned