loading page

Reference range of naïve T and T memory lymphocyte subsets in peripheral blood of healthy adult
  • +10
  • Ying Xia,
  • Aqing Liu,
  • Wentao Li,
  • Yunhe Liu,
  • Guan Zhang,
  • Songshan Ye,
  • Zhijieruo Zhao,
  • Juan Shi,
  • Jianchun Yu,
  • Yingjie Jia,
  • Xu Liu,
  • Yongtie Guo,
  • Huayu Chen
Ying Xia
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Aqing Liu
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Wentao Li
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Yunhe Liu
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Guan Zhang
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Songshan Ye
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Zhijieruo Zhao
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Juan Shi
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Jianchun Yu
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Yingjie Jia
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Xu Liu
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Yongtie Guo
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile
Huayu Chen
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Author Profile

Abstract

Abstract: Background: Naïve T and T memory cell subsets can provide important information for the diagnosis and treatment of immunological and hematological disorders. Lymphocyte compartment undergo dramatic changes during adulthood; age-related reference values derived from healthy individuals are crucial. However, extensively detailed immunophenotyping reference values of peripheral blood lymphocytes in whole spectrum of adulthood performed by flow cytometry-based single-platform method are rare. Methods: 309 healthy adult volunteers were recruited from Tianjin in China. The absolute counts and percentages of CD3+CD4+ T cells, CD3+CD8+ T cells, naïve T cells (Tn), T memory stem cells (Tscm), central memory T cells (Tcm), effector memory T cells (Tem), terminal effector T cells (Tte) were determined by flow cytometry with single platform technologies. Results: Reference range of absolute counts and percentage of lymphocyte subsets were formulated by different age and gender. We also find out the changing regularity of them: the cells which have stem cell properties, Tn and Tscm cells, decrease with aging; memory cell subsets, Tcm and Tem increase with aging, which increase from 18 to 64 years old and present no significant change over the 65 years old. Gender have influence on the fluctuation of lymphocyte subsets, absolute count of CD3+CD8+, CD8+ Tcm, CD8+ Tem in male are higher than that in female. Conclusion: The reference values of percentages and absolute numbers of naïve T and T memory cell subsets can help doctors to understand the immune state of patients and to evaluate conditions of prognosis then adjust treatment for patients.

Peer review status:UNDER REVIEW

09 May 2021Submitted to Clinical & Experimental Immunology
11 May 2021Assigned to Editor
11 May 2021Submission Checks Completed
11 May 2021Reviewer(s) Assigned