loading page

miR399-UBC24 module enhances freezing tolerance through regulating CBF signaling pathway and starch degradation
  • +9
  • Kankan Peng,
  • Yu Tian,
  • Xianze Sun,
  • Chunhua Song,
  • Zhipeng Ren,
  • Yuzhuo Bao,
  • Jinpu Xing,
  • Yuanshan Li,
  • Qinghua Xu,
  • Jing Yu,
  • Da Zhang,
  • Jing Cang
Kankan Peng
Northeast Agricultural University
Author Profile
Yu Tian
Northeast Agricultural University
Author Profile
Xianze Sun
Northeast Agricultural University
Author Profile
Chunhua Song
Northeast Agricultural University
Author Profile
Zhipeng Ren
Northeast Agricultural University
Author Profile
Yuzhuo Bao
Northeast Agricultural University
Author Profile
Jinpu Xing
Northeast Agricultural University
Author Profile
Yuanshan Li
Northeast Agricultural University
Author Profile
Qinghua Xu
Northeast Agricultural University
Author Profile
Jing Yu
Northeast Agricultural University
Author Profile
Da Zhang
Northeast Agricultural University
Author Profile
Jing Cang
Northeast Agricultural University
Author Profile

Abstract

Although the regulation in Pi homeostasis of miR399 have been studied in various plants, its molecular mechanisms in response to freezing stress are still elusive. In this work, we found that the expression of tae-miR399 and its target gene TaUBC24 in tillering nodes of strong cold resistance winter wheat cultivar Dongnongdongmai1 (Dn1) was significantly altered subjected to severe winter. tae-miR399 and its target gene TaUBC24 were also responsive to short-term freezing stress in tillering nodes of Dn1 seedlings. TaUBC24 physically interacted with TaICE1. Ehanced freezing tolerance was observed in overexpressing tae-miR399 Arabidopsis lines. Under freezing stress, overexpressing tae-miR399 decreased the expression of AtUBC24 to increase the expression of genes in CBF signaling pathway, Pi translocation pathway and starch metablism, including AtCBFs, AtCOR47, AtCOR413IM, AtPHT1;4, AtAPLs and AtBAMs, inhibit the degradation of AtICE1 and AtPHO1, and promote the activities of SOD, POD and CAT. These findings indicated that the increased freezing tolerance was dependent upon elevating CBF signaling pathway, phosphorus utilization efficiency, starch degradation, accumulation of soluble sugar and ability of ROS scavenge. These results will aid our understanding of molecular mechanism of how miR399-UBC24 module plays a cardinal role in regulating plant freezing stress tolerance through mediating the downstream pathways.

Peer review status:UNDER REVIEW

05 May 2021Submitted to Plant, Cell & Environment
06 May 2021Assigned to Editor
06 May 2021Submission Checks Completed
16 May 2021Reviewer(s) Assigned