loading page

Co-delivery of Curcumin and Bioperine via PLGA Nanoparticles to Prevent Atherosclerotic Foam Cell Formation
  • +3
  • Sindhu C Pillai,
  • Ankita Borah,
  • Minh Nguyen Tuyet Le,
  • Hiroaki Kawano,
  • Kouichi Hasegawa,
  • D. Sakthi Kumar
Sindhu C Pillai
Toyo University - Kawagoe Campus
Author Profile
Ankita Borah
Toyo University - Kawagoe Campus
Author Profile
Minh Nguyen Tuyet Le
Kyoto University
Author Profile
Hiroaki Kawano
Kyoto University
Author Profile
Kouichi Hasegawa
Kyoto University
Author Profile
D. Sakthi Kumar
Toyo University
Author Profile

Abstract

Cholesterol-rich arterial plaques characterize atherosclerosis, a significant cause of heart disease. Arterial plaques upregulate inflammatory cytokines secreted by the macrophages that finally become cholesterol-laden foam cells. Nutraceuticals have received attention over the years, demonstrating potential benefits towards treating and preventing cardiovascular diseases (CVD), including atherosclerosis. Curcumin, a potent polyphenol present in Curcuma longa, showed remarkable anti-atherosclerotic activity via anti-inflammatory and anti-oxidative properties. The bioavailability and low water solubility of curcumin limit its clinical translational purposes. These issues can be circumvented effectively by nano-drug delivery systems that can target atherosclerotic plaque sites. In this work, we chose to use curcumin and a natural bioenhancer called Bioperine (derived from Piper nigrum) inside a polymeric nano-drug delivery system for targeting atherosclerotic plaque sites. We selected two different ratios of curcumin: Bioperine to study its comparative effect on the inhibition of oxidized low-density lipoprotein (Ox-LDL) induced foam cell formation and investigated the therapeutic efficacy in THP-1 monocyte-derived macrophages via different in vitro cell studies. Our studies demonstrated that Bioperine administration alongside curcumin via PLGA nanoparticles (NPs) imparts a reduction in macrophage-mediated foam cell formation, relative cholesterol content, and the inflammatory pathways when administered as preventive medicine, highlighting the importance of natural-based compounds towards the therapeutic intervention against the atherosclerotic activity.

Peer review status:UNDER REVIEW

11 May 2021Submitted to Biotechnology and Bioengineering
11 May 2021Assigned to Editor
11 May 2021Submission Checks Completed
17 May 2021Reviewer(s) Assigned