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Comprehensive evaluation for the one-pot biosynthesis of butyl-acetate by using microbial mono and co-cultures
  • +6
  • Yang Lv,
  • Yujia Jiang,
  • Jiasheng Lu,
  • Hao Gao,
  • Weiliang Dong,
  • Jie Zhou,
  • Wenming Zhang,
  • Fengxue Xin,
  • Min Jiang
Yang Lv
Nanjing Tech University
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Yujia Jiang
Nanjing Tech University
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Jiasheng Lu
Nanjing Tech University
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Hao Gao
Nanjing Tech University
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Weiliang Dong
Nanjing University of Technology
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Jie Zhou
Nanjing Tech University
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Wenming Zhang
Nanjing University of Technology
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Fengxue Xin
Nanjing University of Technology
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Min Jiang
Nanjing University of Technology
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Abstract

Butyl acetate has shown wide attention in food, cosmetics, medicine and biofuel sectors. These short chain fatty acid esters can be produced by either chemical or biological synthetic process with corresponding alcohols and acids. Currently, biosynthesis of short chain fatty acid esters, such as butyl butyrate through microbial fermentation systems has been achieved; however, few studies regarding biosynthesis of butyl acetate were reported. In this study, three proof-of-principle strategies for the one-pot butyl acetate production from glucose by microbial fermentation was designed and evaluated. (1) 7.3 g/L of butyl acetate was synthesized by butanol producing Clostridium acetobutylicum NJ4 with the supplementation of exogenous acetic acid; (2) With the addition of butanol, 5.76 g/L of butyl acetate can be synthesized by acetate producing Actinobacillus succinogenes130z (ΔpflA) with the supplementation of exogenous butanol; (3) Microbial co-culture of C. acetobutylicum NJ4 and A. succinogenes130z (ΔpflA) can directly produce 2.2 g/L of butyl acetate from glucose, representing the first study in terms of butyl acetate production by using microbial co-culture system. Through the immobilization of A. succinogenes130z (ΔpflA), butyl acetate production was further improved to 2.86 g/L. These strategies may be extended to the biosynthesis of a wide range of esters, especially to some longer chain ones.

Peer review status:UNDER REVIEW

12 May 2021Submitted to Biotechnology and Bioengineering
12 May 2021Assigned to Editor
12 May 2021Submission Checks Completed
22 May 2021Reviewer(s) Assigned