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Impacts of iron on phosphate starvation-induced root hair growth in Arabidopsis
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  • Caiwen Xue,
  • Wenfeng Li,
  • Ren Fang Shen,
  • Ping Lan
Caiwen Xue
Institute of soil science,Chinese academy of sciences
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Wenfeng Li
Nanjing Forestry University
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Ren Fang Shen
Institute of soil science, Chinese academy of sciences
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Ping Lan
Institute of soil science,Chinese academy of sciences
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Abstract

Phosphate is essential for plant growth and development. Root architecture alternations induced by phosphate starvation (-Pi), including primary root and lateral root growth, are mediated by iron (Fe). However, whether and how Fe participates in the -Pi-induced root hair growth (RHG) remains unclear. Here, with morphological, proteomic, and pharmacological analysis, we investigate the impacts of Fe on RHG under -Pi and the underlying mechanisms. We found that -Pi-induced RHG was affected by the local Fe availability. Reduced sensitivity to Fe was found in aux1-7, arf10arf16, and phr1 under -Pi, indicating auxin and phosphate starvation-induced responses were required for the Fe-triggered RHG under -Pi. Fe availability was then found to affect the auxin distribution and expression of phosphate starvation-responsive (PSR) genes. Proteomic analysis indicated vesicle trafficking was affected by Fe under -Pi. With the application of brefeldin A, we found the vesicle trafficking was affected by Fe, and root hairs displayed reduced sensitivity to Fe, indicating the vesicle trafficking is critical for Fe-triggered RHG under -Pi. Our data suggested that Fe is involved in RHG under -Pi by integrating the vesicle trafficking, auxin distribution, and PSR. It further enriches the understanding of the interplay between phosphate and iron on RHG.

Peer review status:UNDER REVIEW

10 May 2021Submitted to Plant, Cell & Environment
11 May 2021Assigned to Editor
11 May 2021Submission Checks Completed
25 May 2021Reviewer(s) Assigned
16 Jun 2021Review(s) Completed, Editorial Evaluation Pending