Investigation of serum isocitrate dehydrogenase, malate dehydrogenase
and glutamate dehydrogenase activities with related oxidative stress
markers in preeclampsia
Abstract
Introduction: Preeclampsia, a high cause of fetomaternal
morbidity-mortality, remains a significant burden affecting 8% of all
pregnancies. Environmental conditions induce disease development leading
to endothelial dysfunction in genetically predisposed women. Our aim is
to discuss oxidative stress as a well-established contributing factor to
disease progression with being the first study to show new evidence
about serum dehydrogenase enzyme levels (isocitrate, malate, glutamate
dehydrogenase) with oxidative markers (myeloperoxidase, total
antioxidant-oxidant status, oxidative stress index). Methods: Serum
parameters were analyzed with photometric method (Abbott ARCHITECT
c8000). Results: Results showed that the enzyme levels and oxidative
markers were significantly higher in patients, supporting the redox
imbalance in preeclampsia. According to ROC analysis, malate
dehydrogenase showed an outstanding diagnostic ability with the highest
AUC value of 0.9 and the cut-off value of 51.2 IU/L. Discriminant
analysis including malate, isocitrate and glutamate dehydrogenase had
predicted preeclampsia with an overall %87.9 accuracy. Discussion:
Considering the above results, we propose that the enzyme levels
increase with oxidative stress functioning as antioxidant defense
factors. The unique finding of the study is that the serum levels of
malate, isocitrate and glutamate dehydrogenase can be used both
separately and combined in the early prediction of preeclampsia. As a
novel approach, we also offer combining serum isocitrate and glutamate
dehydrogenase levels with ALT, AST tests to state liver functions more
reliably in patients. Still, larger sample-sized studies investigating
enzyme expression levels are required to confirm the recent findings and
to reveal underlying mechanisms.