Figure 1. (A) IgA production in supernatants of B cells from 11 healthy donors stimulated (T cell-dependent) for 11 days in the presence of long or short TSLP. (B) IgA production following stimulation with the mucosal factors APRIL, BAFF and IL-10 and/or TSLP for 11 days (3 donors, P>0.05 due to donor variation).(C) T cell-dependent IgG1 and IgA production by B cell (5 donors) stimulated with TSLP and/or type 2 cytokines IL-4 and IL-13.(D-E) Example of FACS staining of live B cells for membrane expression of IgG and IgA after stimulation for 7 days and summary data (N=4-5).