Figure 2. (A) B cells were sorted into naive and memory B cell pools and stimulated using the T cell-dependent protocol for 11 days. Data show IgA and IgG1 production from 2-4 independent replicates from 3 donors. (B-C) Example of flow cytometric analysis for antibody secreting cells (CD38+ CD20-) following stimulation with different stimuli for 7 days and summary data of 3 donors. (D) B cells were cultured for 11 days in the absence or presence of TSLP and from day 5 onwards RA was added to the indicated conditions (N=7).