Introduction
- general info about flu and why people should care
- Explain what is known about the replication dynamics base on (likely) bulk studies
- Explain the limitations of these studies and what they are unable to show
- Introduce the advent of single cell sequencing and how it has helped learn about how individuals behave, even for flu
- Explain why even these single cell RNA-based methods are lacking because they only indicate which genes are expressed, not which gene segments are actually present
- Here, we develop a droplet-based method for sequencing Influenza gene segments in individual cells and apply it to cell line of infected cells. We investigate the genome replication dynamics at unprecedented level of resolution.
Results