Materials and methods
Sodium chloride (Sigma, S7653), Brij O-20 (polyoxyethylene (20) oleyl ether, Sigma, 436240,), Ex-CELL 610-HSF medium (Sigma, 14610C), glycine (Bio-lab 07132391), bathophenanthroline (GFS chemicals, C038446), ferrous chloride tetrahydrate (Sigma, 44939), human IgG (Lee-Biosciences, 340-21), phenylalanine (Sigma, P2126), tyrosine (Sigma, T3754), tryptophan (Sigma, T3300), isoleucine (Sigma, I2752), valine (Sigma, 94640), Polyethylene glycol-6000 (PEG-6000) (Sigma, 81260).