Figure 2: A. SDS-PAGE (Coomassie stained) analysis of process efficiency, extraction at pH 4: Lane 1: Total hIgG added to the system; lane 2: E. coli. lysate added as an artificial contamination background; lane 3: E. coli lysate and total hIgG; lanes 4-9: hIgG recovered via conjugated micellar aggregates comprising: Brij-O20, the [(bathophenanthroline)3:Fe2+] complex and amino acid monomers (see the Materials and Methods section); lane 10: Molecular weight markers (KDa). Red arrows indicate reduced heavy and light chains of hIgG, respectively. B. Process yield when human IgG (input concentration, 5 mg/ml), is extracted at pH 4, with addition of indicated amino acid monomers, while the Control lacks amino acid monomers; C. As in B , but with extraction at pH 6.3. Error bars are calculated as the standard deviation of 4 replicate measurements conducted on different days. Quantitation was accomplished as described in the Materials and Methods section.