1. potential for less biased coverage - good for large surveys to not miss types (brain and beyond)
Single nucleus reflects cell class proportions better - interneuron vs. excitatory and neuron vs. glia? (Lake vs Darmanis)
Single nuclei will enable relatively unbiased sampling of tissue that difficult to dissociate
provide an excellent method for large-scale profiling projects
2. frozen tissue - use banked specimens to study human diversity and disease
For example, snRNA-seq will enable large-scale studies of normal and pathological adult human brain.
Together these results illustrate the high information content of nuclear RNA for cell type classification and characterization, and the high fidelity of snRNA-seq for unbiased characterization of cellular diversity in brain tissues (and likely other tissues).