lack of cell isolation-based transcriptional effects, e.g. IEGs (Wu 2017, Gage/Lasken)
application to archived frozen specimens.
Single nuclei will enable relatively unbiased sampling of tissue that difficult to dissociate and has been frozen, such as banked adult human brain, and provide an excellent method for large-scale profiling projects. For example, snRNA-seq will enable large-scale studies of normal and pathological adult human brain.