2. less expression perturbation - good for linking behavioral studies to circuit function where need a link to cell types via signature of neuronal activity that is masked by cell dissociation (snRNA-seq provides another tool and other techniques are also being developed, e.g. Wu 2017)
3. frozen tissue - use banked specimens to study human diversity and disease
Such as expression of neuronal activity-associated genes that can confound studies linking molecular changes to functional circuits (Wu 2017)
Together these results illustrate the high information content of nuclear RNA for cell type classification and characterization, and the high fidelity of snRNA-seq for unbiased characterization of cellular diversity in brain tissues (and likely other tissues).