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Conserved cell types with divergent features in human versus mouse cortex
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  • Rebecca Hodge,
  • Trygve E. Bakken,
  • Jeremy Miller,
  • Boaz Levi,
  • Jennie Close,
  • Osnat Penn,
  • Ed Lein
Rebecca Hodge
Allen Institute for Brain Science, Seattle, WA, USA
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Trygve E. Bakken
Allen Institute for Brain Science, Seattle, WA, USA
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Jeremy Miller
Allen Institute for Brain Science, Seattle, WA, USA
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Boaz Levi
Allen Institute for Brain Science, Seattle, WA, USA
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Jennie Close
Allen Institute for Brain Science, Seattle, WA, USA
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Osnat Penn
AIBS Molecular Genetics
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Ed Lein
Allen Institute for Brain Science, Seattle, WA, USA
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Abstract

Elucidating the cellular architecture of the human cerebral cortex is central to understanding our cognitive abilities and susceptibility to disease.  Here we applied single nucleus RNA-sequencing to perform a comprehensive analysis of cell types in the middle temporal gyrus of human cortex. We identified a highly diverse set of excitatory and inhibitory neuronal types that are mostly sparse, with excitatory types being less layer-restricted than expected. Comparison to similar mouse cortex single cell RNA-sequencing datasets revealed a surprisingly well-conserved cellular architecture that enables matching of homologous types and predictions of human cell type properties. Despite this general conservation, we also find extensive differences between homologous human and mouse cell types, including dramatic alterations in proportions, laminar distributions, gene expression, and morphology. These species-specific features emphasize the importance of directly studying human brain.