METHODS AND PROCEDURES:
The initial protocol was first initiated with containment of Algae
culture in the Microbial Fuel Cell structure. A Microbial Fuel Cell with
a suitable exchange of Hydrogen Protons and Electrons across the Proton
Exchange Membrane (PEM) was crucial to fully harness the electrochemical
properties of each Algal culture- Chlorella Vulgaris,
Spirulina , and Nannochloropsis. Constant variables were
maintained by precisely quantifying internal conditions and additional
attachments (the PEM). The most basic constants (remaining throughout
complete experimentation) included anodic and cathodic temperatures.
Additionally, the concentrations of Algae culture were regulated at 1 mL
of Algal Culture/10 mL of media. The Algal culture of each genus was
obtained at high concentrations of 8 mL/10 mL of initial media. More
specifically, additional nutrients media was added at concentrations of
1 mg/250 mL. The most critical constant variables and parameters are
listed below, with sufficient range levels to correctly maintain culture
growth: