Table 1.0: Essential (constant) parameters and conditions regulated in the Algal MFC cultures. Parameters are presented with the most optimal ranges/conditions for cultivation. All Algal cultures were continuously maintained within these minimum to maximum ranges.
The most essential procedures included MFC construction, Algae cultivation and maintenance, Electrical testing, and supplementary Oil Yields (optional to experimentation). MFC construction involved attachable/interconnected 450 mL Anode and Cathode containers to fulfill the needs of the Algal culture-Cathode interactions. The Proton Exchange membrane was further composed of an Agar solution brought to boiling point and later froze at 0°C (32°F) . The Cathodic chamber was comprised of an isolated and sterilized (distilled) continuously aerated water. Following the configuration of the Anodic and Cathodic chamber, with the integration of the PEM, MFC underwent electrical testing with a traditional multimeter to detect variations in output levels in each culture chamber. More importantly, direct correlations between Algal concentration and electrical levels (mW) were analyzed and discussed.
MFC Construction: Designing the Anode and Cathode Chambers