The most simplified design of the MFC containment system involved
interlinking both the Anode and Cathode chamber, sandwiched with a
Proton Exchange Membrane. The parameters of both chambers were equalized
to maintain controlled conditions and environments in each container.
Both chambers held a capacity of 450 mL of Distilled water (Cathode) and
Highly Concentrated Algal culture (Anode). In preparation for the PEM,
~10.16 cm diameter circular openings were made,
consistent with the shaping of the circular PEM. The designing of the
PEM system will be further discussed in its specified procedure. The
“suctioning” nature of the Proton Exchange Membrane enabled both the
Anode and Cathode chamber to converge without any major or minor
leakages to interfere with the exchange process. Conveniently, the
purchased tupperware containers were attachable, making reconfiguration
and modification of the system possible. This further enhanced the theme
of interchangeability and attachability, making immediate immediate
configurations to the energy system possible. After nearly 50 mL of
highly concentrated algae culture was obtained, the culture was
immediately transferred to a 500 mL nutrient rich bioreactor medium
composed of an initial 2 mg of All-Purpose growth media (1 mg/250ml).
Parameters, such as cell concentration, pH, aquatic temperature,
salinity, sterilization, and lux levels were assessed. Cell
concentrations were obtained with a spectrophotometer. After two days of
cultivation, the algae was transferred to a 450 mL MFC anode chamber.
Electrical testing was executed with a multimeter.