Elevation of the urinary NAD+ metabolome by oral NR in a 52-year-old male.
A healthy 52-year-old male ingested 1,000 mg NC Cl daily for 1 week. Urine was collected before the first dose, in three collection fractions in the first 12 h and before the second and fifth daily dose. Concentrations (a, Nam; b, MeNam; c, Me2PY; d, Me4PY) are normalized to urinary creatinine. The data indicate that urinary MeNam, Me4PY and Me2PY are the dominant metabolites elevated by oral NR. No phosphorylated species were found in urine.