This often required going beyond traditional teaching methods, using hands-on sessions, interactivity, and problem solving approaches. This will eventually lead to enhancing the course objectives and learning outcomes. In this article, we wish to discuss the challenges and best practices related to delivering training in bioinformatics in lower-limited resource setting. The article also explain how we addressed these challenges we faced in hosting and running this distance-based online introduction to bioinformatics course (IBT) developed by the H3ABioNet Education and Training Working Group and delivered at the University of Khartoum Node.