Sulfamethoxazole is a sulfonamide bacteriostatic antibiotic which is commonly used for the treatment of infections caused by bacteria. In this study, the impact of the Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of sulfamethoxazole was studied using LC-MS and GC-MS spectroscopy. Sulfamethoxazole sample was divided into two parts, one part of sulfamethoxazole was considered as control (no Biofield Energy Treatment was provided), while the second part was received the Consciousness Energy Healing Treatment remotely by a famous Biofield Energy Healer, Dahryn Trivedi and termed as a treated sample. The LC-MS spectra of both the samples at retention time (Rt) 2.5 minutes exhibited the mass of the deprotonated molecular ion peak at m/z 252 [M-H]-. The peak area of the treated sulfamethoxazole was significantly increased by 42.96% compared to the control sample. The LC-MS based isotopic abundance ratio of PM+1/PM in the treated sulfamethoxazole was significantly decreased by 49.56% compared with the control sample. Thus, 13C, 2H, 15N, 17O, and 33S contributions from (C10H10N3O3S)- to m/z 253 in the treated sample were significantly decreased compared with the control sample. The GC-MS peak area% of the treated sample was significantly increased by 80.3% compared to the control sample. The GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated sulfamethoxazole was significantly altered by 119.53% and -25.48%, respectively compared with the control sample. Hence, 13C, 2H, 15N, 17O, 18O, 33S, and 34S contributions from (C10H11N3O3S)+ to m/z 254 and 255 in the treated sample were significantly altered compared with the control sample. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (18O/16O or 34S/32S) in the treated sulfamethoxazole were significantly altered compared to the control sample. It can be assumed that the changes in peak area%, isotopic abundance, and mass peak intensities could be due to changes in nuclei possibly through the interference of neutrino particles via the Trivedi Effect®. The new form of sulfamethoxazole would be more efficacious pharmaceutical formulations that might offer better solubility, dissolution, absorption, bioavailability and therapeutic response against urinary tract infections, tuberculosis, traveler’s diarrhoea, ear infections, shigellosis, bronchitis, and pneumocystis jiroveci pneumonia, etc.