Results

Correlation between fluorescence and plasmid  type frecuency 

(Not done yet)

At population level it has been shown that antibiotics affects the frequency of plasmid types and it correlates with change of fluorescence ratio on our HT strain.
To state that our measures of single cell fluorescence implies an estimate of the plasmid copy number. We used a FACS system to split a population by their levels of fluorescence, once we recovered the cells, we extracted the DNA of each population and submitted it to a qPCR. We made the same experiment to three bacteria populations, one carrying the pBGT plasmid, another carryng the blaTEM1  gene along with the eGFP reporter gene and the wild type as control.
We expect to find that the level of fluorescence correlate with pBGT plasmid copy number.