Antibiotic acts as selective pressure on plasmid fraction at single cell level
(Almost done - tracking)
Using our microfluidic system we performed two different experiments in which we submitted the HT strain to an antibiotic ramp and we found our previously finding that antibiotics change the frequencies of plasmids at population level in fact comes from a change of plasmid frequencies at intracellular level.
In each experiment we let the bacteria grow in LB media for a certain amount of time and then we started to introduce the antibiotic increasing its concentration until it reached an specific inhibitory concentration, IC. We used IC50 for ampicillin and IC25 for ceftazidime.