Antibiotic ramp experiments
Data was taken of HT cells dividing in the chambers under Lb media, then the antibiotic was introduced increasing its concentration until it reached the desired limit under a period of 6 hours. This concentration is kept indefinitely until cells are dead or chambers were empty. For the AMP experiment we used 50% of the inhibitory concentration IC-50, for the CAZ experiment we used IC-25.
Sine experiments
HT cells were observed for about 3 hours and then the antibiotics were introduced. Due to physical setup both antibiotics were introduced at 50% Caz concentration and 50% Amp concentration and follow a sine signal, when sine reaches a maximum, it means that Caz concentration is at 100% and Amp is at 0% and viceversa for the sine signal is at a minimum.
Microscopy
Image acquisition