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\begin{document}

\title{Malaria Rapid Diagnostic Test (RDT): is it possible~to~overcome
limitations?}



\author[1]{Alex Massella}%
\affil[1]{Affiliation not available}%


\vspace{-1em}



  
  \date{May 08, 2024}


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\section*{Background}

{\label{951132}}

Between 2016 and 2017 a ``Differential~ Fever Diagnosis Study (DFDS)''
was conducted in Bafat\selectlanguage{ngerman}à (Guinea Bissau). The aim of the study was to
detect alternative pathogens (bacteria and viruses) which develop a host
response comparable to malaria. For this purpose children under 5 years
showing malaria-like fever were screened and enrolled in the study,
provided that two Plasmodium falciparum RDT's were negative for both
peripheral and venous blood. The samples were than sanded and screened
by real time PCR in a partner Laboratory in Dakar including a 18 S P.
facli to further exclude any presence of the parasite. 16 out of 400
samples resulted positive for the P. falci target~ gene.~~

\par\null\par\null

\section*{Methods}

{\label{589457}}

16 frozen venous blood samples with negative Plasmodium falciparum RDT
but positive by RT-PCR were unfrozen and retested by RDT.~ 12 of them
resulted positive. ~

\section*{Conclusion}

{\label{469707}}\par\null

It is possible to increase the sensitivity of the HRP2 Malaria Rapid
Diagnostic Test for Plasmodium falciparum after freezing and unfreezing
the blood sample to room temperature, allowing the detection of the
parasite before undetectable~by~ RDT's. The main advantage is to reveal
low parasitemia samples of immunized or asymptomatic patients, avoiding
false negative results. Therefore, the already widely used RDT's can be
improved to a certain extent leading to better~ which~

\par\null\par\null

\subsection*{idee}

{\label{593780}}\par\null

\begin{itemize}
\item
  interaction antibody-antigen by Biolayer interferometry
  \cite{Markwalter_2017}
\item
  testing with colture derived P.f. (no antibody) and human venous blood
  samples (with antibody)-\textgreater{} to see if it is
  antibody-antigen interaction or parasitemia to inhibit the reaction
\item
\end{itemize}

\par\null

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