Aging increases cell-to-cell transcriptional variability upon immune stimulation
Methods
Three methods to quantify cell-to-cell variability in gene expression
Test...fsdafsd
Genetic factors affecting EBV copy number in lymphoblastoid cell lines derived from the 1000 Genome Project samples
"Our measures of the proportion of genetic variance explaining EBV inter-individual are highly dependent on the transformation method (population-wise or mixing all populations) and affected by population structure. At least ~3000 individuals, almost twice our current sample size, are recommended as sample size for GCTA to obtain estimates of variance explained with a standard error <0.1 [64]. Therefore, estimates of genetic variance explained from this or previous studies such as [11] should be interpreted with caution."
Host Genetic Variants and Gene Expression Patterns Associated with Epstein-Barr Virus Copy Number in Lymphoblastoid Cell Lines
" The expression of two host genes (CXCL16 and AGL) was positively correlated and expression of ADARB2 was negatively correlated with EBV copy number in a genotype-independent manner. "
Statistical Power to Detect Genetic (Co)Variance of Complex Traits Using SNP Data in Unrelated Samples
scATAC-seq cancer
Nuclei multiplexing with barcoded antibodies for single-nucleus genomics -- solve the problem of population-level scRNA-seq cost
Cell cycle
"Cell cycle assignment was performed using Cyclone (Tables S3 and S4; Scialdone et al., 2015). Gene expression values measured in TPM were used as input. This generated G1, G2M, and S-phase scores for each cell. Cells were assigned to a particular cell cycle stage as previously described (Scialdone et al., 2015) such that if either the G1 or G2M score was above 0.5, the cell was assigned to the stage with the highest score. If neither of these scores was above 0.5, the cell was assigned to S phase."
Integration and Analysis of BRAIN Initiative Data (R01 Clinical Trial Not Allowed) | | Open | March 04, 2021 | - Cell Type
- Circuit Diagrams
- Human Neuroscience
- Integrated Approaches
- Interventional Tools
- Monitor Neural Activity
- Theory & Data Analysis Tools
| This Funding Opportunity Announcement (FOA) solicits applications to develop informatics tools for analyzing, visualizing, and integrating data related to the BRAIN Initiative or to enhance our understanding of the brain. |