Population genetics is the study of variation observed within a population group or among different population groups in terms of allele and genotype frequencies. Great genetic variation exists within groups at the individual nucleotide level. For example, in humans, approximately 10 million nucleotides can differ between individuals. \cite{Barbujani1997}. The modest description of variation is the frequency distribution of genotypes. A degree of this variation is the number of heterozygote individuals observed in a population. Over time, isolated populations diverge from one another, each losing heterozygosity due to inbreeding. This variation on the basis of heterozygosity within and among the populations was first explained by Sewall Wright for univariate model (VNTRs) and then these parameters of differentiation were further explained by Bruce Weir for multivariate models (STRs). These parameters are comprised of co-efficient of inbreeding (FIS or \(f\)), co-efficient of co-ancestry (FST or \(\theta\)) and the co-efficient of relationship (FIT or \(F\)). These indices are well illustrated by Fig 1.