Here are some explanations;
- The red boxes indicate the total number of loci used and the names of each loci (in two separate columns)
- The yellow boxes indicate the total number of samples collected and number of populations
- The green boxes indicate the identifiers (Ind) for individual names and (Pop) for population name columns
- The top green column indicates number of samples collected from each population.
After the data input, the only formatting to do with the data is to multiply all the alleles with 10, This is done to get rid of the decimal point within the entries. The data will still be good enough to represent the alleles as the number of repeats.
Note: In case, if you face problems in installing or using GenAlEx add-in, then it is recommended to go to properties of the add-in file and Unblock or add it into the trusted sites of Excel.
Forensic statistics analyses
After formatting the input file, we are ready to perform the statistical analyses on the available datasets. First thing to start with, is the calculation of allele frequencies of all the populations and making separate databases of each population.
Allele frequency calculations
In order to calculate the allele frequencies of each population, we will perform the commands in this sequence;
GenAlEx > Analysis Options > Frequency Based > Frequency
Initiating this analysis, a window will appear, displaying the brief information of the input file. On verifying this information (by clicking OK) another window will pop-up. Please, ensure that the "Frequency by pop" and "Graph All Loci" options are checked, and then click OK. The output of this analysis will give the allele frequency of each locus in all the studied populations.
In order to calculate other statistical parameters of forensic interest, please go to;
Data Management > Import-Export > Export > GenePop
Please, uncheck the "To Text File" and click OK. The participants are required to save this file with a \(.gen\) extension with the name.
FORSTAT Application
Please go to the homepage of FORSTAT server, available here \cite{forstat} and upload the saved file with \(.gen\) extension (Load and view data) and Perform computations (Compute) and once the computations are complete then click Output.
On the left, you will have the access to all the options to perform further detailed tests of forensic importance and explore the data.
In the last option, you will have the possibility of downloading all the statistics and graphs.
Population statistics analyses
Package installation
Now the participants are requested to open RStudio and install all the required packages, by running this command.