Determination of Experimental Variables

In order to understand how photo-current is obtained from a GE/P(DTP-Ph-NH2)/Cyt C/thylakoid membrane electrode, several control experiments were carried out. First, the modified electrode was put into ethanol instead of water, and treated with 6800 W/m2 of light in the visible range of the spectrum, but no any considerable photo-current formation was observed. This experiment revealed that the system is sensitive only to water, and that it generates photo-current through the electron transfer as a result of the photosynthesis-caused oxidation of water. Furthermore, before the whole electrode fabrication, the thylakoid membrane was only immobilized onto the electrode surface and measured the photo-bioelectrochemical activity.