marker strongly linked to the Polima male sterility. Also we found new candidate genes linked to Ogu CMS based on SNP difference using Ogu CMS population. The objectives of this study is to develop additional molecular markers and candidate genes linked to the Rfp and Rfo genes in B. napus and other Brassica species. This information will help to determine the Rfp and Rfo genotype in heterosis breeding system and to achieve map-based cloning of these gene.