\textbf{sourcecode} \href{https://github.com/Arktthul/Measuring-the-membrane-porosity-on-microalgae-Under-progress/blob/master/MicroAlgues_Part01.ijm}{< MicroAlgues_Part01.ijm>}  

sourcecode : part 1

2. Step 2 :  Segmentation

 Segmentation occurs in two major steps. Preparation of the MembraneChannel and Segmentation itself.

1. As the membrane labelling can have a noisy background, we realise this series of action

    Duplicate the MembraneChannel image
    Run a gaussian blur
    Realise the substraction of MembraneChannel by this new image we called BackgroundCleanup. 
    Close the image to save space
    Rename the result of subtraction to be called ForSegmentation.