Table.1 Blue-white screen for verification of plasmid transformation into E.coli on LB plates containing carbenicillin. Transformation procedure was conducted using CaCl2/heat shock method. White colonies indicated bacteria containing recombinant plasmid and blue colonies indicated bacteria containing empty vectors. T4 DNA ligase ligated taq DNA to vector plasmid pETBlue-1. Positive control was transformed with supercoiled pETBlue-1 and negative control was not transformed with any DNA. Recombination frequency was calculated as the percentage of white colonies and transformation efficiency was calculated as the number of cells transformed per 1 μg of plasmid vector.